Effect of Four Different Media on Periodontal Ligament Cells Viability of Dry- Stored Dog Teeth

STATEMENT OF THE PROBLEM The maintenance of viable periodontal ligament cells is the most important issue in the long-term preservation of avulsed teeth. PURPOSE The aim of this study was to assess aloe vera as a new storage media in maintaining the cell viability of dry-stored teeth in comparison with soy milk, Hank`s balanced salt solution (HBSS), and milk. MATERIALS AND METHOD Twenty one extracted dog premolar teeth were dried for 30 minutes and stored in soy milk, HBSS, milk, and aloe vera extract (50%) for 45 minutes (n=6 for each). Furthermore, positive and two negative control groups (n=6), corresponding to 0 min, 30 min, and 2-hour drying times were also prepared respectively. The number of viable cells was counted following storage using Trypan blue exclusion. Data were statistically analyzed using the one-way ANOVA and post hoc Tukey-HSD test. RESULTS Statistical analysis showed no significant differences in cell viability among aloe vera, soymilk, and HBSS- stored teeth; however, they were all superior to milk. CONCLUSION Aloe vera extract can be recommended as a suitable storage media for avulsed teeth.